FIG. 3.

HPLC analysis of hydrolyzed products from PGA by YwtD. PGA and the hydrolyzed products were analyzed by HPLC using an Asahipack GF-7 M HQ (300 by 7.6 mm; Showa Denko Co., Tokyo, Japan) with a refractive index detector. Samples were eluted with 50 mM phosphate buffer (pH 6.8) at a flow rate of 0.6 ml/min at 32°C. Peaks were assigned by coelution with authentic pullulan with a molecular mass of 200 kDa (Tokyo Kasei Co, Tokyo, Japan) and α-l-PGAs with molecular masses of 58, 32, and 14 kDa (Sigma).