TABLE 1.
Analytical data for PGA and products hydrolyzed by YwtD
| Polymer | Proportion in total PGA (%) | Avg molecular mass (kDa)a | Composition of glutamic acid (%)
|
Hydrolysis by carboxypeptidase G (%)b | |
|---|---|---|---|---|---|
| d-Isomer | l-Isomer | ||||
| PGA | 100 | 1,500 | 70 | 30 | 8.21 (0.4) |
| F-1 | 18 | 490 | 0 | 100 | 24.5 (1.2) |
| F-2 | 82 | 11 | 80 | 20 | 0.0 (0) |
a
The apparent molecular masses of PGA and the hydrolyzed products were measured by gel filtration HPLC as described in the legend to Fig. 3.
b
Values are the nanomoles of free glutamic acid under the conditions described in the text. Percentages are quantitative ratios of free glutamic acid to each polymer.