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(A) Pyrophosphate exchange by M. maripaludis LysRS. The reaction was incubated for 20 min at 37°C in the absence of various components as indicated. Values represent micromoles of ATP in a 20-μl aliquot. (B) In vitro Ap4A biosynthesis. Reactions were performed in the presence of E. coli cell-free extract (•), M. maripaludis LysRS (○), or B. burgdorferi LysRS (■). Values represent picomoles of Ap4A in a 50-μl aliquot.
