Table 3.

Recognition of tRNA^Lys by B. burgdorferi LysRS

tRNALys	B. burgdorferi LysRS Vmax/KM, relative*	E. coli LysRS Vmax/KM, relative†
Wild-type	1	1
Anticodon
UCU	<0.04	<0.01
UAC	<0.04	<0.01
UUA	<0.04	<0.01
UUG	0.08	0.22
GUU	0.34	0.059
Discriminator
G73	0.28	0.11
C73	0.28	0.10
U73	0.29	0.12

^*For the wild-type tRNA^Lys, k_cat = 0.22 ± 0.03 min⁻¹ and K_M = 1.5 ± 0.6 μM. 

^†Values taken from ref. 33 (for wild-type, k_cat = 3.32 min⁻¹ and K_M = 1.9 μM).