Table 4.

Recognition of in vitro transcribed tRNA^Lys variants by B. burgdorferi and E. coli LysRS

LysRS	tRNA	KM tRNA, μM	kcat, min−1	kcat/KM, R*
B. burgdorferi	B. burgdorferi	1.98 ± 0.32	47.4 ± 4.2	1
	E. coli wild type	1.5 ± 0.6	0.22 ± 0.003	160
	E. coli G2⋅U71	3.9 ± 0.6	0.21 ± 0.02	432
E. coli	B. burgdorferi	4.3 ± 0.4	0.21 ± 0.01	490
	E. coli wild type†	1.9	3.32	14
	E. coli G2⋅U71	6.3 ± 0.8	0.15 ± 0.008	1022

^*Relative value expressed as fold decrease in k_cat/K_M compared to aminoacylation of B. burgdorferi tRNA₁^Lys transcript by B. burgdorferi LysRS. 

^†From ref. 33.