FIG. 1.
Different binding properties of wild-type and mutant OxyR to unmethylated agn43 regulatory region. Results obtained from EMSA with cell extracts containing OxyR are shown in panel A (lanes 2 to 5), with OxyR(H198R) in panel B, and with OxyR(A233V) in panel C. Amounts of total protein added were 0 μg (lanes 1 and 6), 0.42 μg (lanes 2), 0.85 μg (lanes 3), 2.55 μg (lanes 4), and 4.25 μg (lanes 5). Also shown is the shift obtained with 7 ng of purified, oxidized OxyR (A, lane 7).