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. 2003 Apr;185(7):2203–2209. doi: 10.1128/JB.185.7.2203-2209.2003

FIG. 2.

FIG. 2.

Oxidized OxyR represses transcription from an unmethylated agn43 template in vitro. Template DNA was either unmethylated (lanes 1 to 3) or methylated (lanes 4 to 6). OxyR(ox) was added as indicated before RNA polymerase to a final concentration of 9.5 nM (lanes 2 and 5) or 19 nM (lanes 1 and 6). No OxyR was added to reactions shown in lanes 3 and 4. “Control” refers to a transcript obtained from a vector-derived promoter. The size of the agn43 transcription product is 220 nt. Lane 2 was obtained from a different exposure of the same gel.

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