FIG. 3.
The DNase I footprint of oxidized OxyR is similar to that of OxyR(C199S) at agn43 DNA. Footprints were obtained using a final concentration of 12 nM OxyR(ox) (lanes 1 and 4) or 10 nM OxyR(C199S) (lanes 3 and 6). Template DNA was unmethylated agn43 DNA (lanes 1 to 3) and oxyRS DNA (lanes 4 to 6). For the agn43 sequence, the position of the three GATC sequences is shown. The direction of transcription of oxyR and oxyS is shown for the oxyRS footprint, as well as the position of the OxyR binding motifs O1 through O5, as designated previously by Toledano et al. (34).