TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype and/or phenotype | Reference or source |
|---|---|---|
| E. coli | ||
| DHB6521 | Δlac mel gyrA supF λInCh1 (Kmr) | 3 |
| VJS632 | F− λ− prototroph | 30 |
| VJS676 | As VJS632 but Δ(argF-lacIZYA)U169 | 30 |
| Derivatives of strain VJS676 (λRS45-derived lysogens) | ||
| VJS6959 | λ [O3-lac O1-lac lacZ+Y+A+] | This study |
| VJS6934 | λ [O3-lac O1-nrfA lacZ+Y+A+] | This study |
| VJS6311 | λ [O3-lac O1-nirB lacZ+Y+A+] | This study |
| VJS6313 | λ [O3-lac O1-nirB lacZ+Y+A+]/narL215::Tn10 | This study |
| VJS6315 | λ [O3-lac O1-nirB lacZ+Y+A+]/narP253::Tn10d(Cm) | This study |
| VJS6317 | λ [O3-lac O1-nirB lacZ+Y+A+]/narL215::Tn10 narP253::Tn10d(Cm) | This study |
| VJS6813 | λ [O3-lac O1-napF lacZ+Y+A+] | This study |
| VJS6814 | λ [O3-lac O1-napF lacZ+Y+A+]/narL215::Tn10 | This study |
| VJS6815 | λ [O3-lac O1-napF lacZ+Y+A+]/narP253::Tn10d(Cm) | This study |
| VJS6816 | λ [O3-lac O1-napF lacZ+Y+A+]/narL215::Tn10 narP253::Tn10d(Cm) | This study |
| VJS6945 | λ [O3-lac O1-fdnG lacZ+Y+A+] | This study |
| VJS6978 | λ [O3-lac O1-fdnG lacZ+Y+A+]/narL215::Tn10 | This study |
| VJS6979 | λ [O3-lac O1-fdnG lacZ+Y+A+]/narP253::Tn10d(Cm) | This study |
| VJS6980 | λ [O3-lac O1-fdnG lacZ+Y+A+]/narL215::Tn10 narP253::Tn10d(Cm) | This study |
| Derivatives of strain VJS676 (λInCh-derived segregants) | ||
| VJS7450 | λ− Δ(attλ-lom)::bla [O3-lac O1-lac lacZ+] | This study |
| VJS7445 | λ− Δ(attλ-lom)::bla [O3-lac O1-nirB lacZ+] | This study |
| VJS7484 | λ− Δ(attλ-lom)::bla [O3-lac O1-fdnG lacZ+] | This study |
| VJS7489 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+] | This study |
| VJS7906 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/narL249::Ω-Sp | This study |
| VJS7907 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/narP253::Tn10d(Cm) | This study |
| VJS7905 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/narQ251::Tn10d(Tc) | This study |
| VJS7911 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/narQ251::Tn10d(Tc) narL249::Ω-Sp | This study |
| VJS7912 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/narQ251::Tn10d(Tc) narP253::Tn10d(Cm) | This study |
| VJS7904 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/ΔnarX242 | This study |
| VJS7909 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/Δ(narXL)235 | This study |
| VJS7910 | λ− Δ(attλ-lom)::bla [O3-lac O1-nrfA lacZ+]/ΔnarX242 narP253::Tn10d(Cm) | This study |
| VJS7449 | λ− Δ(attλ-lom)::bla [O3-lac O1-napF lacZ+] | This study |
| VJS7475 | λ− Δ(attλ-lom)::bla [O3-lac O1-napF lacZ+]/narL215::Tn10 | This study |
| VJS7476 | λ− Δ(attλ-lom)::bla [O3-lac O1-napF lacZ+]/narP253::Tn10d(Cm) | This study |
| Plasmids | ||
| pRS414 | Apr ′lacZ gene fusion vector | 28 |
| pVJS3253 | Apr Δ(lacY lacA cynX tet) derivative of pRS414 | This study |