TABLE 5.
Effects of narX, narQ, narL, and narP null alleles on expression of the lacZ gene from the O3-lac O1-nrfA constructa
| Genotype
|
LacZ sp actb with:
|
Repression with:
|
||||||
|---|---|---|---|---|---|---|---|---|
| narL | narP | narX | narQ | No addition | NO3− | NO2− | NO3− | NO2− |
| + | + | + | + | 6,660 | 60 | 470 | 110 | 14 |
| + | − | + | + | 6,460 | 65 | 1,140 | 100 | 5.7 |
| − | + | + | + | 6,580 | 180 | 340 | 36 | 19 |
| + | + | + | − | 6,180 | 90 | 1,590 | 69 | 3.9 |
| + | − | + | − | 6,500 | 90 | 1,520 | 72 | 4.3 |
| − | + | + | − | 3,420 | 520 | 660 | 6.6 | 5.2 |
| + | + | − | + | 6,840 | 40 | 60 | 170 | 110 |
| + | − | − | + | 6,700 | 60 | 60 | 110 | 110 |
| − | + | − | + | 6,580 | 50 | 60 | 130 | 110 |
a
Specific activity is indicated in Miller units.
b
Strains were cultured overnight in MOPS defined medium with limiting glucose. All are λInCh-derived segregants.