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UV induces p48 transcription by a p53-dependent pathway. Primary human fibroblast cell lines, either p53+/+ (WI38) or p53−/− (041 mut), were treated with 10 J/m2 UV radiation and were harvested after different time intervals. The level of p53 protein was measured by immunoblot (IB) of protein extract. The levels of p48, p21, p125, actin mRNA, and 28S rRNA were measured by Northern blot (NB) of total RNA. The levels of 28S rRNA confirmed equal transfer of total RNA to the membrane.
