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. 1999 Jan 19;96(2):424–428. doi: 10.1073/pnas.96.2.424

Figure 3.

Figure 3

Expression of p53 induces p48 transcription and the activity of UV-damaged DNA binding protein. We used p53−/− (041 mut) cells that had been stably transfected with a control vector not containing p53 (p53−) or with a vector containing wild-type p53 cDNA under the control of a tetracycline regulated promoter (p53+) (2). Cells were analyzed for levels of p53 protein, p48, p21, p125, actin mRNA, and 28S rRNA at different times after inducing p53 expression by removal of tetracycline. UV-DDB was measured by an electrophoretic mobility-shift assay (EMSA) with UV-damaged DNA probe.