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In vitro osteoclastogenesis is not attached to ChM-I inactivation. Bone marrow macrophages were isolated from tibial bone marrow of 12-week-old mice and were induced to osteoclast formation by stimulation with 100 ng of M-CSF/ml and 100 ng of soluble rhRANKL/ml as described in Materials and Methods. TRAP-positive multinucleated cells (MNCs) per well were counted. Data are means ± standard deviations for four wells. WT, wild type; KO, knockout; N.S., not significant.
