TABLE 3.
Effect of menin missense mutations on interaction with RPA2a
| Menin | β-Galactosidase activity
|
|
|---|---|---|
| RPA2 | JunD* | |
| Wild type | ++ | +++ |
| P12L | +/− | +++ |
| L22R | ++/+ | +++ |
| H139D | − | − |
| H139Y | + | − |
| F144V | +/− | +++ |
| F159C | ++/+ | +++ |
| A160P | − | − |
| A164D | ++ | +++ |
| A176P | − | − |
| W183S | ++ | − |
| L286P | + | ++ |
| A309P | +/− | ++ |
| T344R | ++/+ | + |
| W436R | ++ | +++ |
| F447S | ++ | +++ |
a
Bait proteins consisting of Gal4 DNA-binding domain fusions with wild-type menin or the indicated menin missense mutants were tested simultaneously for interaction with prey proteins consisting of Gal4 activation domain-RPA2 or -JunD* fusions as described in Table 2, footnote a. +, ++, and +++, low, moderate, and high levels of β-galactosidase activity, respectively; −, no activity. A level of activity that falls between two categories is indicated by a combination of two symbols separated by a slash.