FIG. 4.
Effect of the mitochondrial complex III inhibitor antimycin A on fibroblast fatty acid β-oxidation. Human and mouse fibroblasts from wild-type (WT) and affected individuals were grown for 2 days in medium containing antimycin A (0.4 μg/ml). Wild-type LCFA β-oxidation (C16:0) and VLCFA (C24:0) fatty acid β-oxidation were determined in each experiment as described in Materials and Methods and set to 1. These values were used to calculate the fold decrease in activity in the fibroblast cell lines being studied. Results are shown as means ± the standard deviations with the number of experiments performed in parentheses.