FIG. 5.
Effect of 4PBA on fatty acid metabolism in vivo. VLCFA accumulation (A) and β-oxidation (B) were determined in liver postnuclear supernatants from wild-type (WT) mice, ALD mice, and ALD mice treated with 4PBA (0.16 g/kg/day) or TSA (0.3 mg/kg/day) in water. Total lipids were extracted, fractionated, and analyzed by gas chromatography as previously described (20). Wild-type mouse C26:0/C22:0 ratios and fatty acid β-oxidation activity levels were set to 1, and these values were used to calculate changes in ratios and activity levels in untreated and treated ALD mice. Results are shown as means ± the standard deviations with the number of experiments performed in parentheses.