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. 2003 Jan;23(2):744–753. doi: 10.1128/MCB.23.2.744-753.2003

FIG. 8.

FIG. 8.

Effect of 4PBA (5 mM) on mitochondrial mass in human X-ALD fibroblasts. Mitotracker Green FM was used to measure mitochondrial mass in fibroblasts by flow cytometry before and after 4PBA treatment. The autofluorescence of unstained cells (A) was analyzed and subtracted from the analysis of fibroblasts stained with Mitotracker before (B) and after (C) 4PBA treatment. The 2.4-fold increase in mitochondrial mass after 4PBA treatment is shown in panel D. SSC, single scanned cell. Results are shown as means ± the standard deviations with the number of experiments performed in parentheses.