FIG. 1.
The effects of conditioned media on MTC cells. TT cells were treated with the conditioned medium Raf-E2-CM and the control conditioned media pLNCX-EtOH-CM, pLNCX-E2-CM, and Raf-EtOH-CM. Cells were observed for changes in morphology (A), growth (B), incorporation of [3H]thymidine into DNA in 6 h (C), and expression of phosphorylated ERK1/2 and RET (Western blotting) or calcitonin genes (Northern hybridization) after 2 days (D). Data (mean ± standard error) are from a representative experiment performed in triplicate. P value is <0.001 for Raf-E2-CM compared to Raf-EtOH-CM on a cell growth curve (one-way analysis of variance). Experiments were repeated at least three times with similar results.