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. 2003 Jan;23(2):620–628. doi: 10.1128/MCB.23.2.620-628.2003

FIG. 1.

FIG. 1.

(A) IFN-induced transcription is enhanced by expression of Mediator subunits. Expression vectors for the DRIP proteins indicated (2 μg) were transfected with ISRE-luciferase, and cells were treated for 6 h with 1,000 U of IFN-α/ml. Extracts were normalized for total protein. Values shown are the averages plus standard deviation (SD) from triplicate experiments. (B) Dose-dependent enhancement of ISRE-luciferase activity by DRIP150 expression. Cells were transfected with reporter genes and increasing amounts of DRIP150 expression plasmid as indicated. Dose-dependent enhancement was observed until a point where inhibitory effects were observed, presumably due to squelching by saturation of Mediator complexes at higher DRIP150 levels. Extracts were normalized for total protein. Values shown are averages plus SD from triplicate experiments.