Figure 4.

Association of Fyn with PSD-95 in mouse synaptosomes. Synaptosomes from wild-type (wt) or fyn-mutant (fyn) mice were lysed in TNE buffer. The lysates were immunoprecipitated with goat anti-NR2A, anti-Fyn, anti-Src, anti-Lyn, or anti-Yes antibody. These antibodies were not crossreactive (data not shown). The immunoprecipitates (IP) and lysates were subjected to immunoblotting with anti-PSD-95 antibody. All experiments were performed more than three times. Positions and sizes (kDa) of standard protein markers are indicated. The positions of PSD-95 are indicated by arrowheads.