FIG. 3.

C6 glioma cell lines constitutively overexpressing T-cadherin exhibit decreased cell proliferation. (A) Western blot demonstrates overexpression of the mature (105-kDa) and precursor (130-kDa) forms of T-cadherin in stably transfected C6 cell clones T3, T9, and T13. (B) Direct cell counting demonstrates that T-cadherin-expressing C6 cell clones T3, T9, and T13 have decreased cell proliferation compared to vector-transfected clones V2 and V3 on day 7 of culture. The means and SDs are shown for each time point. Error bars, SDs. (C) T-cadherin-overexpressing C6 cell clones (T3, T9, and T13) formed fewer colonies in soft agar than vector-transfected C6 cell clones (V2 and V3). The single asterisk denotes a statistically significant decrease in colony number of T-cadherin clones compared to vector clones. Error bars, SDs.