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. 2003 Jan;23(2):462–473. doi: 10.1128/MCB.23.2.462-473.2003

FIG. 4.

FIG. 4.

p53 function is increased independently of p53 accumulation in mdm2puro/Δ7-12 thymocytes. (A) Northern analysis of p21 and gapdh mRNAs in multiple tissues. (B) Northern analysis of p21 and gapdh mRNAs in the spleen and thymus in the presence and absence of p53. (C) Western analysis of p53, p21, and BAX in isolated thymocytes. MEFs lacking p53 were used as the negative control. TBP was used as a loading control, and increasing amounts of lysate from wild-type MEFs were used as standards. (D) Western analysis of p53 prior to and following DNA damage. Mice were left unirradiated (−IR) or subjected in duplicate to 10 Gy of ionizing radiation (+IR). Four hours after irradiation, thymocytes were isolated. Increasing amounts of lysate from wild-type MEFs were used as standards. (E) Western analysis of p53 following deletion of exons 7 through 9 of mdm2 in spleens of mdm2flox/Δ7-12 Mx-cre+ mice.