FIG. 6.

A defect in 60S synthesis affects regulation of ribosome synthesis even when it does not affect growth rate. Cultures of strains J1203 (wild type) and J1204 (rpl11bΔ) were grown in YPGE at 30°C, in which they have the same doubling time, for about 4 h. Samples were taken before and 4 h after treatment with 2.5 μg of tunicamycin/ml. RNA was prepared and subjected to Northern analysis. The presence of tunicamycin reduced the RP mRNA by about 50% in wild-type cells but not at all in cells carrying rpl11bΔ.