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. 2003 Jan;23(2):733–743. doi: 10.1128/MCB.23.2.733-743.2003

FIG. 5.

FIG. 5.

Repair of a DSB by allelic recombination does not result in detectable levels of LOH of markers located ≥1 cM from the DSB. The genotypes of the chromosome 14 SSLP markers D14Mit160, D14Mit224, and D14Mit95 were analyzed by PCR amplification using primers that flank each polymorphism and resolution of the products on high-percentage agarose gels. The genotypes of the D14Mit160 and D14Mit224 markers were determined for 160 recombinants. The genotype of the D14Mit95 marker was determined for the 17 recombinants with LOH at the H3 marker and for an additional 15 recombinants without LOH at this marker. The amplified products for the 129 and BALB/c alleles are indicated. S/P, parental cell line; BALB, BALB/c mouse; R, a representative recombinant clone.