Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
cdc6K114E fails to initiate S phase but inhibits mitosis. Asynchronous cultures of K4055, YB504, and YB506 were grown to exponential phase in SC medium lacking methionine. Methionine was added to 2 mM to repress the wild-type pMET3-CDC6 present in all strains, and time points were processed for FACS analysis. From left to right, strains encode no CDC6, or either cdc6K114E or cdc6ΔN, K114E integrated at the LEU2 locus and under the control of the CDC6 promoter.
