Table 1.
Strains
| Strain | Genotype | Source |
|---|---|---|
| W303-1A | Mata leu2-3,112 trp1-1 ura3-1 his3-11,15 can1-100 ade2-1 | R. Rothstein |
| K4055 | W303 Mata cdc6Δ∷hisG pMET3-CDC6∷TRP1 | K. Nasmyth |
| K6210 | W303 Mata MCM7(Myc7)-URA3 | " |
| YB209 | W303 Mata cdc6Δ/pRS426-CDC6 | (10) |
| YB495 | W303 Matα cdc6Δ/pRS426-CDC6 | This study |
| YB496 | YB209/pMW71 | " |
| YB497 | YB209/pMW126 | " |
| YB502 | K4055 CDC6∷LEU2 | " |
| YB503 | K4055 cdc6K114A∷LEU2 | " |
| YB504 | K4055 cdc6K114E∷LEU2 | " |
| YB505 | K4055 cdc6DE(223,224)AA∷LEU2 | " |
| YB506 | K4055 cdc6ΔN,K114E∷LEU2 | " |
| YB507 | YB209 CDC6∷LEU2 | " |
| YB508 | YB209 cdc6K114A∷LEU2 | " |
| YB510 | YB209 cdc6DE(223,224)AA∷LEU2 | " |
| YB511 | W303 Mata dbf2-1 MCM4(T7)-HIS3 MCM6(HA3)-LEU2 MCM7(Myc7)-URA3 | " |