FIG. 3.

The Sin3-Rpd3 complex is required for proper rapamycin-induced repression of RP genes. (A) Isogenic wild-type (MLY41a), and rpd3 (JRY16a), sin3 (JRY17a), and sap30 (JRY18a) mutant strains were grown to early exponential phase and treated with or without 200 ng of rapamycin ml⁻¹ for 15, 30, and 60 min as indicated. RNA was prepared and analyzed by Northern blotting with radioactive probes that hybridize to the genes indicated at the left. (B) Following the rapamycin treatment as indicated above in Fig. 3A, cultures were cross-linked with formaldehyde. Chromatin was prepared and immunoprecipitated with antibodies specific for hyperacetylated histone H4. PCR was performed in the total chromatin (Input) or the immunoprecipitated DNA with specific primers for the RPL9A promoter. The results shown are representative of two independent experiments.