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. 2003 Jan;23(2):526–533. doi: 10.1128/MCB.23.2.526-533.2003

FIG. 3.

FIG. 3.

CBP/p300 proteins are required for M. tuberculosis induction of TNF-α. (A) Inhibition of CBP/p300 impairs TNF-α gene induction by M. tuberculosis. J774 cells were cotransfected with 1 μg of the −200 TNF-α luciferase reporter and with 2 μg of the vectors expressing wild-type E1A 12S or mutant (Δ2-36) forms of E1A 12S as indicated. Following transfection, cells were treated with M. tuberculosis whole sonicate for 16 h. Data are presented as means ± standard errors of the means (SEM) of three independent experiments. (B) M. tuberculosis potentiates the transcriptional activity of CBP and p300. J774 cells were cotransfected with a Gal4-dependent luciferase reporter (Gal4x5-luc, 1 μg) and increasing amounts of vectors expressing full-length CBP or full-length p300 fused to the Gal4 DNA-binding domain (0.02, 0.07, 0.2, 0.7, or 2 μg) or the Gal4 DNA-binding domain alone (2 μg). Transfected cells were then treated with M. tuberculosis whole sonicate for 16 h (Mtb) or mock stimulation (UN). Data are presented as means ± SEM of three independent experiments. DBD, DNA-binding domain.