Figure 3.

Real-time PCR analysis of ORP mRNA expression during monocyte-macrophage differentiation. Monocytes isolated from two healthy individuals (A and B) were differentiated into macrophages by incubation for 4 or 8 d in serum-containing medium. Total RNA was isolated from monocytes and the differentiated macrophages. Real-time PCR analysis of the messages for ORP1L, ORP1S, nine other ORP genes, OSBP, and GAPDH was performed with the Amplifluor-system using an ABI7700 analyzer. Expression levels on day 4 (gray bars) and on day 8 (black bars) are given relative to that of monocytes. The data has been normalized using the total amount of RNA in each sample. Data are given as mean ± SD (n = 2).