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. 2003 Mar;14(3):903–915. doi: 10.1091/mbc.E02-08-0459

Figure 4.

Figure 4

ORP1L and ORP1S proteins in human brain and during monocyte-macrophage differentiation. Proteins were resolved in 12.5% SDS-PAGE and Western blotted with ORP1-specific antibodies. The cell and tissue specimens are indicated above the lanes. ORP1L and ORP1S refer to nontagged transiently overexpressed proteins in CHO-K1 cells, the MOCK lane was transfected with empty vector pcDNA3.1. R250 refers to the primary antibody against amino acids 428–950 in ORP1L, common to both ORP1 variants. R279 refers to the primary antibody against an N-terminal peptide consisting of amino acids 118–137, present only in ORP1L. The mobilities of ORP1L and ORP1S are indicated on the right with an arrow and an arrowhead, respectively. Molecular weight markers are indicated on the left.