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Electron microscopy of endosome morphology in cells stably transfected with ORP1L cDNA (ORP1L) or the empty vector pcDNA3.1 (MOCK). The cells were fixed with glutaraldehyde, processed for electron microscopy using a standard procedure, and horizontal sections were viewed with an EX200 electron microscope (JOEL). The ORP1L-expressing cells display abundant multivesicular body/late endosome-like organelles that are abnormally full of electron dense internal membranes. Bar, 1 μm.
