Table 2.

Primers used for quantitation of ORP mRNA expression levels

Forward primer (5′→3′)		Reverse primer (5′→3′)		Size (bp)	E value
ORP1L-F	TGATTGCCTTAATCTCTTCACC	ORP1-R	aACTCAGGGACCTTTCGGACTC	223	1.78
ORP1S-F	GGTCCTCGGATCTGGCCCA	ORP1-R	aACTCAGGGACCTTTCGGACTC	209	1.88
ORP1LS-F	GTGAGGAATTTTAAATTGGAACAA	ORP1-R	aACTCAGGGACCTTTCGGACTC	189	1.57
ORP2-F	aACCACCTGAGAAAGGCCAAGC	ORP2-R	CTCCAGCTCGTTGAGGCTCAC	197	1.65
ORP3-F	aGCATCTAGCTACTACCGAGCT	ORP3-R	CACATGGGTTGTGCCAATTGGA	237	1.58
ORP4-F	aAAGCTCTGGATCGACCAGTCA	ORP4-R	CTGGGACTGCTATGCATGACC	218	1.72
ORP5-F	aGTGCCGCTGGAGGAGCAGAC	ORP5-R	AGGGGCTGTGGTCCTCGTATC	235	1.69
ORP6-F	aGGATACTGCTCCACCTATTTCA	ORP6-R	ACAGGCAGGATTTCCATCGAC	227	1.53
ORP7-F	aCGGCCTACTCCTCCACATACC	ORP7-R	ACTGTTCCCACAGGCACAATC	238	1.49
ORP8-F	aGAAGAACAGGGAGATTTTGAATCA	ORP8-R	TCCTGTGAGTGGATCAAGTTC	192	1.54
ORP9-F	aCATCTTCCACACTAAACCCTTC	ORP9-R	CTCGTTCTGATCTTCCAACTTC	217	1.58
ORP10-F	aCTCAGCGACAGTGATATTCCAC	ORP10-R	AGGTCTGATCTTCTTGGGATAC	208	1.56
ORP11-F	aCACATTTTCTCTACCCTGTGCA	ORP11-R	CCCTTGCACTCTGCATACCAC	211	1.65
OSBP-F	aGATCCATCAGGAAAAGTCCAC	OSBP-R	CAGTGCCACTTTCCCAAGCA	235	1.61
GAPDH-F	CGGAGTCAACGGATTTGGTCGTAT	GAPDH-R	aAGCCTTCTCCATGGTGGTGAAGAC	307	1.56

^a Primer carries UniPrimer target sequence 5′-ACTGAACCTGACCGTACA-3′ at its 5′ end. The sizes of the PCR products are given without this 18-bp extension. Real-time PCR efficiencies for each target gene are shown in the E value column.