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. 2003 Mar;14(3):939–957. doi: 10.1091/mbc.E02-10-0633

Figure 3.

Figure 3

Temperature-sensitive growth of Pex3p variants. (A) Levels of the wild-type and mutant c-Myc-tagged Pex3 proteins expressed in transformed pex3KOA cells are comparable. “No insert” refers to the pex3KOA strain containing the vector pINA445 alone. Lysates of the various strains were subjected to immunoblotting with the 9E10 antibody against the c-Myc epitope. The immunodetection of Kar2p was used as a loading control. (B) Mutant MycPex3 proteins R143P and L320P confer ts growth on oleic acid-containing agar to the pex3KOA strain. The days of incubation at 30°C or 16°C are indicated. Numbering of strains is as in A.