Table 1.

Y. lipolytica strains used in this study

Strain	Genotype
E122	MATA, ura3-302, leu2-270, lys8-11
22301-3	MATB, ura3-302, leu2-270, his1
mut12-1	MATA, ura3-302, leu2-270, lys8-11, mut12-1
M12TR	MATA, ura3-302, leu2-270, lys8-11, p12(LEU2)
pex3KOA	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3
pex3KOB	MATB, ura3-302, leu2-270, his1, pex3∷URA3
D-WT	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1
D3-1	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1, mut12-1/+
D3-2	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1, +/pex3∷URA3
D3-3	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1, pex3∷URA3/+
D3-4	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1, mut12-1/pex3∷URA3
D3-5	MATA/MATB, ura3-302/ura3-302, leu2-270/leu2-270, lys8-11/+, +/his1, pex3∷URA3/pex3∷URA3
Myc-3lnt	MATA, ura3-302, leu2-270, lys8-11, pex3∷Myc-PEX3
PMyc-3	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3(LEU2)
PMyc-3Q36P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3Q36P(LEU2)
PMyc-3R143P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3R143P(LEU2)
PMyc-3L162P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3L162P(LEU2)
PMyc-3L226P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3L226P(LEU2)
PMyc-3L320P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3L320P(LEU2)
PMyc-3E415Stop	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3E415Stop(LEU2)
PMyc-3Y424Stop	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3Y424Stop(LEU2)
PMyc-3TH-WT	MATA, ura3-302, leu2-270, lys8-11, pMyc-PEX3TH(URA3)
PMyc-3TH-12	MATA, ura3-302, leu2-270, lys8-11, mut12-1, pMyc-PEX3TH(URA3)
PEYFP-SKL-WT	MATA, ura3-302, leu2-270, lys8-11, pEYFP-SKL (HygB)
PEYFP-SKL-PMyc-3R143P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3R143P(LEU2), pEYFP-SKL (HygB)
PEYFP-SKL-Pmyc-3L320P	MATA, ura3-302, leu2-270, lys8-11, pex3∷URA3, pMyc-PEX3L320P(LEU2), pEYFP-SKL (HygB)