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. 2003 Mar;14(3):1017–1026. doi: 10.1091/mbc.E02-01-0034

Figure 3.

Figure 3

Dgp71WD interacts with Dgrips84, 91, 128, and 163. Dgp71WD was coexpressed with Flag-tagged Dgrip84 (A), Flag-tagged Dgrip91 (B), untagged Dgrip128 (C), or Flag-tagged Dgrip163 (D) in Sf9 cells. Antibodies against Dgp71WD (A–D), Flag (A, B, and D), and Dgrips84 (A), 91 (B), 128 (C), and 163 (D) were used to immunoprecipitate the respective proteins from the cell lysates. The immunoprecipitated proteins were fractionated by SDS-PAGE followed by either Coomassie Blue staining (A–D, top panels) or Western blotting (A–D, bottom panels). Antibody indicates the heavy and light chains of the antibody molecules on the Coomassie Blue–stained gels. The heavy chain of the Flag mAb migrated slower than the heavy chains of the rabbit polyclonal antibodies. Asterisks indicate contaminating proteins present in the immunoprecipitations.