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. 2003 Mar;14(3):1017–1026. doi: 10.1091/mbc.E02-01-0034

Figure 4.

Figure 4

Dgp71WD interacts with γ -tubulin. (A and B) Dgp71WD and γ-tubulin were coexpressed in Sf9 cells and then immunoprecipitated with antibodies against Dgp71WD (71), γ-tubulin (γ-tub), or nonimmunized rabbit IgG (NR). The immunoprecipitates were fractionated by 10% SDS-PAGE and then analyzed either by Coomassie Blue staining (A) or by Western blotting, probing with the antibody against Dgp71WD and the mAb against human γ-tubulin (Sigma). (C) Quantification of the expression levels of Dgp71WD and γ-tubulin. Dgp71WD and γ-tubulin were either expressed alone or together in Sf9 cells. The cell lysates were analyzed by Western blotting and probing with antibodies against Dgp71WD and γ-tubulin. (D) Two antibodies against γ-tubulin, DrosC, and Dγ2, the rabbit nonimmunized IgG (NR), and the antibody against Dgp71WD were used for immunoprecipitation of the lysates in C. The immunoprecipitates were analyzed by Western blotting and probing with antibodies against Dgp71WD and the mAb against human γ-tubulin.