Figure 6.

Splicing factors are functionally competent upon entry into daughter nuclei. RNA fluorescence in situ hybridization was performed on HeLa cells stably expressing a β-tropomyosin minigene. The locus is detected during interphase as a single dot (c, arrow) that recruits splicing factors such as SF2/ASF (b, arrow). During telophase, SF2/ASF (e) and B" (h) are recruited to the locus, which is detected by a splice junction probe (SJ) (f and i), demonstrating that splicing occurs at this stage. Control hybridization of 12-mer oligonucleotides (l) shows no hybridization signal at loci decorated with SF2/ASF (k, arrow indicates probable transcription site). DNA was stained with DAPI (a, d, g, and j). Bar, 5 μm.