Figure 8.

Live cell observations of entry of SF2/ASF into daughter cell nuclei. HeLa cells were transiently transfected with YFP-SF2/ASF and live-cell observations were initiated 2 d posttransfection. Metaphase cells exhibit one to two MIGs near the metaphase plate (a, arrow). As the cell enters anaphase, MIGs become more abundant (b and c). SF2/ASF begins to enter daughter nuclei at telophase (d). Nuclear entry of YFP-SF2/ASF is nearly complete in ∼20 min (h). Time is indicated in min from the initiation of imaging the metaphase cell (see video, Mitosis.mov).