Figure 2.

Localization of dynamin 2 and invadopodia markers to EDS. A375MM melanoma cells were plated on cross-linked fluorophore-conjugated matrix for 15 h, and then fixed, stained with anti-dynamin (Dyn2-ab), anti-cortactin, or anti-phosphotyrosine antibodies or phalloidin-TRITC and analyzed at the confocal microscope. ECM degradation patches (A, D, and G, arrows) from substrate level optical sections clearly coincided with either cortactin (B), actin (E), or phosphotyrosine (H) and a reinforcement of the Dyn2 signal (C, F, and I). Bar, 10 μm.