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Effects of dominant negative dynamin 2 mutants on EDS. A375MM cells transiently transfected with wtDyn2aa-GFP (A–C), Dyn2(aa)ΔPRD-GFP (D–F), or Dyn2(aa)K44A-GFP (G–I) were cultured as described above, fixed, stained with anti-phalloidin-TRITC, and analyzed at the confocal microscope. Substrate level optical sections show the fluorescent matrix (A, D, and G), actin staining (B, E, and H), and the GFP signal (C, F, and I). Bar, 10 μm.
