Figure 5.

Dynamics of invadopodia. A375MM melanoma cells, transiently transfected with wtDyn2aa-GFP, were plated on cross-linked fluorophore-conjugated matrix for 15 h and then analyzed at the confocal microscope at 37°C. (A) Digital capture of the cell imaged in the experiment shown: a single EDS (boxed area) was bleached for 30 s and the fluorescence allowed to recover for 80 s at 0.5 frames/s. FRAP is plotted as percentage of FRAP in time (B).