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Fine structures of the conditional spo14-7 mutants at a restrictive temperature. RM14 and RM14-7 were grown to a midlog phase at 30°C and then shifted to the restrictive temperature (20°C). After incubation for 4 h, cells were fixed for electron microscopy. Arrowheads and arrows represent invagination of plasma membranes and the ER-like structures, respectively. A, RM14; B and C, RM14-7. D, E, and F, higher magnified images of A, B, and C, respectively. Bars, 1 μm.
