Figure 3.

Analysis of procyclin expression (A) Western blot analysis of whole cell lysate from wild-type EATRO 795, Δgpi8 and Δgpi8[GPI8] detected with α-EP procyclin, α-GPEET procyclin, or α-aldolase. (B) Wild-type EATRO 795, Δgpi8, and Δgpi8[GPI8] were hypotonically lysed, and the washed pellet was subjected to CHAPS extraction. After SDS-PAGE, CHAPS extracted protein was detected with Stainsall or SyproRuby. Arrows indicate abundant proteins present in all three samples (←) or predominantly in the wild-type EATRO 795 and Δgpi8[GPI8] or Δgpi8 (→).