Table 1.
Sample | T, °C | F0, nN | t0, ×103 s | τ, ×103 s |
---|---|---|---|---|
Cell adhesion | ||||
Phil/serum | 23 | 121 | 2.4 | 3.7 |
Phil/serum | 37 | 189 | 0.0 | 3.2 |
Phil/BSA | 23 | 121 | 1.7 | 3.8 |
Phil/BSA | 37 | 184 | 0.64 | 3.2 |
Phil/FN | 23 | 183 | 2.2 | 4.9 |
Phil/FN | 37 | 204 | 0.86 | 1.1 |
Phil/LAM | 23 | 108 | 1.7 | 1.9 |
Phil/LAM | 37 | 105 | 0.72 | 0.66 |
Phob/serum | 23 | 0.0 | — | — |
Phob/serum | 32 | 101 | 1.6 | 11.9 |
Phob/serum | 37 | 87 | 1.9 | 4.7 |
Phil/no serum | 23 | 45 | 1.7 | 1.7 |
Phil/no serum | 37 | 45 | 0.44 | 0.63 |
Phob/no serum | 37 | 37 | — | — |
Phil/BSA, no serum | 37 | 19 | — | — |
Microsphere adsorption | ||||
Phil/clean/FN-sphere | 23 | 186 | — | — |
Phob/clean/FN-sphere | 23 | >220 | — | — |
Phil/BSA/FN-sphere | 23 | 2.3 | — | — |
Phob/BSA/FN-sphere | 23 | 0.27 | — | — |
Median forces were fit to the adhesion model to obtain the saturation force F0, the lag time t0, and the attachment time τ. Samples with no t0 or τ reported were allowed to reach the saturation force before measurement, so only the median force is reported. Each sample is described by the substrate used, where Phil is hydrophilic polystyrene for cell culture and Phob is hydrophobic polystyrene.