Figure 2.

(a) Time-dependent accumulation of BODIPY Fl-paclitaxel in cells and lumens of isolated rat brain capillaries in the absence and presence of 1 mM NaCN. Means ± SEM, n = 10–12, P = 0.05. (b) Accumulation of BODIPY Fl-paclitaxel and NBDL-CS (c), respectively, in the lumens of isolated capillaries in the absence and presence of unlabeled paclitaxel, the p-gp blocker valspodar, and the mrp2 substrate LTC₄. The fluorescent compounds were added at concentrations of 1 μM. Means ± SEM, n = 10–12. *Significantly different from controls; P = 0.05.