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. 1999 Jan 19;96(2):487–492. doi: 10.1073/pnas.96.2.487

Table 1.

Rhodopsin yields from HEK293S suspension growth (0.5 liter) under different medium conditions

Medium DMEM base cCaCl2/μM Wet cell mass/g⋅L−1 Yield rhodopsin/mg⋅L−1
Standard 1,360 + FBS* 1.7 1.54
Custom made 100 + FBS* 3.1 1.77
Custom made 170 + dialyzed FBS 3.2 1.04
Custom made 340 + dialyzed FBS 4 1.78
*

Includes roughly 140 μM CaCl2 contribution from FBS.