Figure 4.
KC/CXCL1 and MIP-2/CXCL2/3 mRNA levels are markedly elevated during VILI. (a) RT-PCR determination of KC/CXCL1 mRNA in lung homogenates from mice placed on either high– or low–peak pressure/stretch ventilator protocol, or from normal nonventilated mice. Semiquantitative results are expressed as a ratio of each PCR product to β-actin band density (n = 4 mice per group). *P < 0.02. (b) RT-PCR determination of MIP-2/CXCL2/3 mRNA in lung homogenates from mice placed on either high– or low–peak pressure/stretch ventilator protocol, or from normal nonventilated mice. Semiquantitative results are expressed as a ratio of each PCR product to β-actin band density (n = 4 mice per group). *P < 0.02.