Figure 3.

Northern blots of mouse claudin-3, -4, -5, -7, and -8 expression. Mouse Multiple Tissue Northern Blot (CLONTECH) was probed with DNA fragments of claudins (see Materials and Methods). Tissue distribution patterns were distinct between claudin species. The expression of claudin-6 was not detected by Northern blotting, although it was detected by reverse transcription–PCR at least in mouse kidney.