Figure 5.

Subcellular distribution of endogenous claudin-3/mRVP-1, claudin-4/mCPE-R, and claudin-8. (A) Specificity of anticlaudin-3, -4, and -8 pAbs. Immunoblots of total lysates of E. coli expressing GST fusion proteins with cytoplasmic domains of all claudin family members (arrow) confirmed their specificities. CBB, Coomassie brilliant blue staining. (B) Frozen sections of mouse liver (a and b) and kidney (c–f) were stained with affinity-purified anti-claudin-3 pAb (a), anti-claudin-4 pAb (c), or anti-claudin-8 pAb (e). All sections were counter-stained with anti-occludin mAb (b, d, and f). Arrows, distal tubules; asterisks, proximal tubules. (Bar = 10 μm.)