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IL-6 stimulated phosphorylation of Akt in a dose- and time-dependent manner in 7TD1 cells. 7TD1 cells were starved in IL-6-free medium for 6 h. Then the cells were cultured in the presence of different dose of IL-6 for 5 min or in the presence of 1 ng/ml IL-6 for 0, 5, 10, 30, 60 min respectively. After the cells were collected and washed, whole-cell extracts were prepared and subjected to Western-blotting assay. This figure is representative of 3 separate experiments.
